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The purpose of this workshop is to acquaint petroleum industry and environmental professionals with the tools, methods, terminology, and interpretive techniques of stable isotope geochemistry used to understand the origin and fate of hydrocarbons and associated compounds in the earth’s subsurface. Since, in practice, stable isotope geochemical data must be constrained by equally important geological, hydrological, and engineering data, a large portion of the workshop will be dedicated to interpreting isotope data in conjunction with other earth science information. The workshop will provide a general overview of first principals concerning stable isotope geochemistry in the earth sciences, followed by in-depth discussions and exercises about natural gas and liquid hydrocarbon geochemistry, and stray gas investigation and mitigation. The workshop is designed for geologists, geophysicists, engineers, and environmental professionals interested in gaining a basic knowledge of the applications of isotope geochemistry in petroleum geoscience, especially with regard to exploration, production, and environmental problems commonly encountered by the oil and gas industry in its search for energy resources.